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To elucidate the intracellular signal transduction pathways of human prolactin (PRL) in breast cancer, we performed cDNA microarray analysis of MCF-7 breast cancer cells exposed to PRL for up to 60 min and compared the profiles to those in cells stimulated with epidermal growth factor (EGF). Both PRL and EGF increased the levels of growth associated protein-43 (GAP-43) in MCF-7 cells; PRL, however, stimulated not only GAP-43 but also the phosphatidylinositol 3-kinase (PI3K) signaling pathway. Stimulation with EGF, on the other hand, resulted in induction of a number of genes, including the PI3K-related genes, suggesting that EGF exerts a more diverse role in the signal transduction pathway. These results indicate that PRL can activate the PI3K pathway in human breast cancer cells. in comparison with the control according to Antonov et al.[@B4] and the difference can be attributed to the samples being rehydrated in water at room temperature rather than at boiling water temperature.
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