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Activate Windows 10 using Powershell Commands How to install Windows 10 If your Microsoft Windows 7 or above PC has problems with the Internet Explorer 11 the easiest way to.Arachidonic acid metabolites during maturation of the human fetal lung.
Mucus production in the lung epithelium is an important component of the pathophysiology of premature birth. It was hypothesized that arachidonic acid metabolites that mediate mucus production in other epithelia were important for the homeostasis of premature lung epithelium. To test this hypothesis, we characterized the abundance and production of arachidonic acid metabolites during the maturation of the human fetal lung. Fetal lung explants were cultured to various developmental stages and production of [14C]-arachidonic acid metabolites were assayed by thin-layer chromatography. The predominant arachidonate metabolites generated were eicosanoids, including PGE2, 5-HETE, and LTC4. All three arachidonate metabolites were generated in small amounts in the lungs as early as 13-15 weeks’ gestation. Production of eicosanoids increased during development and peaked in the preterm stage of 22-24 weeks, while the production of PGE2 decreased. The amount of leukotriene generation increased during development. Since eicosanoid generation has been associated with mucus production in epithelia other than the lung, we examined the effects of inhibitors of arachidonate lipoxygenase (phenidone) and 5-lipoxygenase (esculetin) on mucin production in the fetal lung. These two inhibitors reduced mucin production and the overall abundance of mucus-producing cells in the lungs by 43-50%. This suggests that these arachidonic acid metabolites mediate mucus production in the fetal lung.//
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