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Men’s Health participates in various affiliate marketing programs, which means we may get paid commissions on editorially chosen products purchased through our links to retailer sites.This proposal outlines plans to test the hypothesis that a series of surface macromolecules on the surface of mammalian cell nuclei mediate an association of nuclear membranes with the cytoplasmic surface of the cell. The approach will be to establish the general nature of the association and then determine the structural and functional significance of this interaction. The principal hypothesis to be tested is that the nuclear membranes are bound to the cytoplasmic surface of the nucleus via a series of membrane glycoproteins that form part of the macromolecular structures of the envelope surrounding the nuclei. This proposal outlines a plan to isolate such membrane glycoproteins in an attempt to characterize their structure and function. The isolation of these glycoproteins should permit a detailed study of their roles in membrane-cytoplasmic interactions in mammalian cell nuclei. In the proposed studies the cells will be isolated in membrane-free forms and the membranes prepared by the procedures of the applicant. The nuclei will be fractionated, and the membranes extracted from both intact and disrupted nuclei. This will permit a determination of the cellular location of the membrane glycoproteins. In addition, the surface proteins of isolated membranes will be investigated by radiolabeling and autoradiographic procedures and characterized by biochemical analysis. The proteins will also be isolated, and the structure of the native molecules determined using two-dimensional gel electrophoresis.It’s a Matt Skube day for Michigan
Notre Dame quarterback Matt Skube, Michigan defensive end/linebacker Ryan Van Bergen and Notre Dame defensive tackle Nick Vannett have been named consensus All-American. Skube’s Wolverines will face the Irish in the BCS National Championship.
As predicted, Michigan was chosen as one of the top seven BCS schools.
Other schools that have won multiple national titles: USC, Nebraska, LSU, Oklahoma and Florida.
Notre Dame did what was expected and won the BCS title. Skube
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