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Two biodegradable, transfection-enhancing, polycationic polymers are being investigated for use in DNA transfection. These are (1) polyamidoamine (PAMAM) dendrimers modified with lysine moieties at the surface, and (2) branched, DNA-binding polymers terminated with poly(ethylene glycol) (PEG) molecules. Previous work has shown that these polymers, based on either charged or cationic moieties, can dramatically increase the efficiency of transfection of a wide range of mammalian cell types when used as gene delivery vehicles. Several laboratories have examined these polymers to optimize their transfection activity, as
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The purpose of this study is to evaluate the accuracy of a novel technique for assisted precision reaming by comparison with the best match in the computer with a medium femur model. Reaming accuracy was determined by measuring lengths of lines on radiographs. Line length measurements were recorded and compared with those obtained with an established technique. The results showed that the novel technique yielded similar accuracy with better consistency, resulting in higher degree of reproducibility. Therefore, the clinical application of the novel technique was demonstrated by comparison of the results obtained with different surgeons.Tris(2-pyridylmethyl)amine (TPA) as an inhibitor of nitric oxide and carbon monoxide synthesis in rat peritoneal macrophages and in activated human polymorphonuclear leukocytes.
Tris(2-pyridylmethyl)amine (TPA) has recently been reported to inhibit the production of nitric oxide (NO) and carbon monoxide (CO) in activated rat peritoneal macrophages and activated human polymorphonuclear leukocytes. The purpose of this study was to determine whether NO and CO production by rat peritoneal macrophages and activated human polymorphonuclear leukocytes (PMN) was inhibited by TPA in doses that only reduced cell viability by less than 10%. Treatment of activated rat peritoneal macrophages or activated human PMN with TPA (25-100 microM) caused a dose-dependent reduction in NO and CO production in a macrophage- and a PMN-dependent manner. The percentage of cells still viable was not affected by TPA
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